Emerging evidences have demonstrated that gold nanoparticles (AuNPs) have been used for cancer treatment. The aim of this\nstudy was to investigate the effects and molecular mechanisms of AuNPs on papillary thyroid carcinoma (PTC) cells (BCPAP\nand TPC-1). Characterizations of AuNPs were detected by UV-Vis spectra, transmission electron microscopy (TEM), and\ndynamic light scattering (DLS). Cell proliferation and apoptosis, migration, and invasion of PTC cells were evaluated by MTT,\nflow cytometry, wound healing, and transwell assays, respectively. Furthermore, qRT-PCR and western blot assays were\nperformed to assess the protein expressions related to apoptosis and migration including caspase-3, caspase-9, Bax, Bcl-2,\nMMP-2, and MMP-9. The study revealed that AuNPs significantly suppressed cell viability, migration, and invasion and\nremarkably induced apoptosis of BCPAP and TPC-1 cells compared with the control group. Moreover, AuNPs negatively\nregulated the expression of CCT3 and silencing of CCT3 obviously promoted the proliferation, migration, and invasion\ninhibition and apoptosis induction of PTC cells combined with AuNPs. Collectively, these results highlighted the potential\napplication of AuNPs in PTC target therapy.
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